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Restoration experiments then introduce exogenous glutathione or precursors and measure recovery of redox markers, enzyme activity, and viability parameters, establishing causal relationships between GSH levels and cellular outcomes
Animals were allowed to recover for 48 h to measure persistent long-lived DNA damage by IHC 58 , after which, the submandibular glands were harvested, fixed, sectioned, and analyzed using immunohistochemistry
Accordingly, inhibition of the main enzyme (asparagine synthetase- ASNS) involved in asparagine biosynthesis can also restrain tumor cells development/proliferation by multiple mechanisms, including (i) weakening Nucks1 effects in the proliferation of osteosarcoma cells
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