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The third EPS secretion mechanism, which appears to be used by bacteria for the secretion of many high-molecular-weight polysaccharide moieties, such as cellulose 45 , alginate 46 , and poly--D- N -acetylglucosamine (PNAG 47 ), is called the synthase-dependent pathway 7 , referring to the fact that a cytoplasmic membrane-embedded glycosyl transferase simultaneously facilitates polymerization and trans-membrane translocation 48