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The cells were lysed and the total ATP content was quantified according to the manufacturers instructions using ATP standard curves obtained from medium spiked with ATP standard solutions (one calibration curve for each medium)

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Recombinant HGF was treated only with LASSS (at the same 1:8000 molar ratio to HGF as described earlier, without exposure to peroxynitrire) and then applied to centrifugal ultra-filtration (10-kDa MWCO) to thoroughly wash out un-reacted, free LASSS (FW: 238.39) prior to the c-met binding assay

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