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J Clin Endocr Metab 104:568580 Schomburg L, Schweizer U (2009) Hierarchical regulation of selenoprotein expression and sex-specific effects of selenium

Store the GSH standard solution at -20C, the solution is stable for 2 months

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We drew a standard curve using the gradient dilution of the standard product as a template, added the sample, antibody and HRP aggregation reagent, and put it in a 37C water bath, then washed it five times with a washing solution, we added the chromogenic solution and reacted for 10 min, then detected the absorbance of each hole at a wavelength of 450 nm