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Try switching injection sites from abdomen to lateral thigh or outer arm, where subcutaneous fat distribution differs
27 These conditions, including the antibodies used and the recombinant human Trx1 standards utilized, impacted accuracy of measurement and contributed to huge differences in Trx1 levels between studies, making between-study comparisons very difficult
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To measure iron concentration in whole-cell lysates, pelleted cells were lysed in optimized 2 RIPA lysis buffer (20 mM Tris-HCl, pH 7.4, 300 mM NaCl, 0.2% SDS, 0.1% sodium deoxycholate, 2% Triton X-100, 0.1 M HCl) for 20 min on ice, followed by centrifugation at 10,000 g for 10 min at 4 C
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