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Intestinal IL-22 and total GLP-1 levels were subsequently measured using the OptEIA Mouse IL-22 Set (R&D Systems, Minneapolis, MN, USA) and Multi Species GLP-1 Total ELISA Bulk Kit (Merck)

References Bergmann, N

Confluent cell cultures were washed once with PBS, and then incubated for 20 min at room temperature with NucBlue Live ReadyProbes Reagent (#R37605, ThermoFisher Scientific, Waltham, MA, USA) to stain the nuclei, washed with PBS, permeabilized with 0.1% Triton-X 100 for 30 min, fixed at 37 C for 20 min with 4% Paraformaldehyde in PBS, and blocked with 2% BSA in PBS at room temperature for 20 min

They also may prescribe treatment to help manage certain side effects if appropriate