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An equal volume of cell lysis buffer containing PCR buffer, detergents (NP-40, Sigma, I8896

These proteins are the target element for molecules that regulate TJ function, and are therefore responsible for the regulation of permeability of the intestinal barrier [18, 19] (Fig

Fluorescence intensity measured in negative controls (samples without BHZ incubation) was averaged and subtracted from sample measurements to adjust for background in the bar graphs