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By week 52, PINP levels had returned to their initial values in all groups ( Fig
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To purify FBU-His-EEHV-EE2, cell pellets were incubated in lysis buffer (20 mM Tris-HCl, pH 7.8, 0.5 M NaCl, 10 mM imidazole, 6 M guanidine hydrochloride (GdmCl)) for 15 min on ice
Those who resume old eating patterns without the appetite-suppressing benefit typically regain weight more rapidly
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