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Further studies revealed that GLC increases melanin production in melanocytes by three pathways, 1) activating activator protein-1 (AP-1) and CRE promoter to activate p42/44 MAPK signaling, 2) activating cAMP signaling, and 3) decreasing GSK3 phosphorylation while inducing CREB phosphorylation (Lee et al., 2005)

Age represents a significant variable, as older adults naturally experience sarcopenia (age-related muscle loss) and may have reduced capacity for muscle protein synthesis

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Standard handling practices include: Following structured laboratory protocols helps support formulation stability and analytical consistency throughout observation periods