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In a second round of dual selection, proteases were evolved under a more stringent selection that lowered the amount of available positive selection substrate and increased the concentration of gIII -neg (Extended Data Fig

[10] GPX1 Glutathione peroxidase reduces hydrogen peroxide radicals (H2O2) and lipid peroxides to water and lipid alcohols and in turn oxidizes glutathione to glutathione disulfide

By maintaining healthier cellular function, it contributes to both internal wellness and visible aesthetic improvements