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Then, standard enzymatic assays were performed in 100-mM sodium phosphate buffer (containing 1 mM EDTA, pH 6.5), 1 mM GSH (Sigma-Aldrich), and 1 mM 1-chloro-2,4-dinitrobenzene (CDNB) (Sigma-Aldrich) at 30 C
Certain health conditions, such as pernicious anemia or specific gastrointestinal disorders that affect B12 absorption, may require even longer-term or lifelong monthly injections
Simultaneously, higher S-adenosylmethionine (SAM) availability supports key methylation-dependent pathways, which are crucial for epigenetic regulation and gene expression related to energy metabolism
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