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The neuronal pathological changes were also observed in the acquired digital images saved as uncompressed 24-bit RGB TIFF files in the software program AnalySIS (Olympus Soft Imaging System GmbH, Mnster, Germany) performing quantitative analysis of neuronal damage in the karyopyknotic areas
Then pENTR/U6-shRNA plasmids were recombined with the Gateway based pAd-BLOCK-iT DEST vector (Thermofisher) to generate pAd shRNA vector
White WM, Makin IRS, Barthe PG, Slayton MH, Gliklich RE
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