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You do not need to be in a fasted state, nor do you need to have just eaten a large meal

doi:10.1146/annurev.biochem.77.032207.120833

It activates melanocortin receptors in the central nervous system, the pathways tied to sexual desire and arousal, so it influences the signal for desire itself rather than the mechanics of circulation

For deuterium tracing, the following day the media was changed to DMEM media containing 10% FS or 10% dFBS supplemented with 100 M acetate and 10% deuterium oxide (Sigma 151882)