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The PVDF membrane was subsequently washed (5 min 5) with tris-buffered saline with tween (TBST) buffer and then incubated with the appropriate secondary antibody (Peroxidase-conjugated goat anti-rabbit IgG (H + L), at a dilution of 1:5000) in a 5% bovine serum albumin solution for 1 h at 25 C

To elaborate, PD-associated proteins like parkin, LRRK2, and DJ-1 have been reported to activate microglia (5), as well as molecules released by damaged dopaminergic neurons such as neuromelanin, - synuclein, and active form of matrix metalloproteinase 3 (MMP-3) (9)

Bacteriostatic water has its own 28-day shelf life after first access

doi: 10.1016/j.bbagen.2012.10.012 199 Mosquera-SulbaranJ