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Mice used in Kir6.1 membrane localisation study were given an anaesthetic overdose (pentobarbital sodium, 200 mg/kg, i.p.) at the end of 10 min reperfusion and transcardially perfused with 20 ml saline, followed by 5 ml 4% PFA for fixation, the hearts were fixed overnight in 4% PFA and sliced into 150 m transverse sections
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