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Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26

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doi:10.1007/s00204-012-0924-1 Hinson JA, Roberts DW, James LP (2010) Mechanisms of acetaminophen-induced liver necrosis