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In further studies, only the formation of [14C]carbon dioxide from [14C-1]glucose was measured, with the addition of: sodium ascorbate (which undergoes a non-enzymic reaction in air to produce hydrogen peroxide) acetylphenylhydrazine (which is known to precipitate haemolysis in sensitive subjects, and depletes glutathione) methylene blue (which oxidises NADPH) All incubations were repeated in the presence and absence of N -ethylmaleimide, which undergoes a non-enzymic reaction with the -SH group of reduced glutathione, and thus depletes the cell of glutathione
Fluorescent protein-based redox probes
Image: Peter Dazeley/Getty Images About the Author Marygrace Taylor, Health Writer About the Reviewer Pieter Cohen, MD, Contributor Disclaimer: As a service to our readers, Harvard Health Publishing provides access to our library of archived content
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