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Jul 16 2021;10(14)doi:10.3390/jcm10143151

Heavy alcohol use

Following the incubation, the cells were washed twice with 1X PBS and DCFDA fluorescence intensity was determined at 491 nm excitation and 516 nm emission using fluorescence microplate reader

Its involvement adds an epigenetic layer to neuronal vulnerability, potentially driving the progressive neuronal loss observed in conditions such as AD [154, 155]