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In another study, semaglutide exhibited greater potency in activating GLP-1R compared to GLP-1, with an EC50 of 6.2 pM for semaglutide vs 16.2 pM for GLP-1 (95) (The variability in EC50 values among different studies for GLP-1 was not uncommon attributable to varying assay conditions)

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The assessment of free testosterone and DHEAS concentrations was performed by a commercial enzyme-linked immunosorbent assay) ELISA) kit (Abbott Healthcare Pvt