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Following synthesis, the crude peptide undergoes purification using reverse-phase high-performance liquid chromatography (RP-HPLC) to remove synthesis-related impurities and isolate the target compound
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Next, OCR and ECAR profiles in response to injection of glucose (25 mM), oligomycin (2 M), carbonyl cyanide- 4 -(trifluoromethoxy) phenylhydrazone (FCCP) (0.3 M), and a combination of antimycin A (1 M) and rotenone (1 M) were evaluated
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