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Heterogenous participants can be another limitation

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Complete loss of expression of H3K27me3 was observed in 72% of malignant peripheral nerve sheath tumors compared with 37% of loss found in melanomas, including primary desmoplastic/fusiform cell melanomas, indicating that complete loss of H3K27me3 is not specific for malignant peripheral nerve sheath tumor in the differential diagnosis, especially with desmoplastic/fusiform cell melanomas