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According to the instructions of the Annexin V-FITC Apoptosis Detection Kit (Beyotime, C1062), 5 L of Annexin V-FITC reagent was added and gently mixed, followed by the addition of 10 L of propidium iodide staining solution, which was also gently mixed
On the contrary, these metabolites were not produced by the rats receiving the isotope intravenously and germ-free rats receiving the tracer orally, suggesting that orally ingested LC is in part degraded by the guts microorganisms [55]
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