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At 48-hour post-treatment, the cell culture supernatant was collected and tested on Human IFN gamma ELISA Kit (Invitrogen), Human IL-2 ELISA Kit (Acro Biosystems), and Human TNF alpha Uncoated ELISA Kit (Invitrogen) to measure IFN-, IL-2 and TNF-, respectively, according to the manufacturer instruction
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However, the levels of LH, FSH, and testosterone significantly increased in both MSG + LC 100 and MSG + LC 200 groups in comparison to the MSG group
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