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Freezing and thawing successively lead to tissue disruption and hence to a better release of folate
Requires medical supervision and dose adjustments
a , HT-1080 transfected with pBP GFP-cGPX4 WT , pBP GFP-cGPX4 R152H , pBabepuro (pBP) empty vector, pBP GFP-cGPX4 K48A , pBP GFP-cGPX4 K48L and pBP GFP-cGPX4 K125R-K127R were selected with puromycin and imaged with microscope
In the direct comparison of iNs_D14 to iNs_D7, we observed the most substantial upregulation in glycerophospholipid metabolism, consistent with ongoing membrane remodeling during neuronal maturation, while glycolysis and gluconeogenesis pathways were downregulated (Online Resource 1Fig
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