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And I'm cautious with anyone who has had bariatric surgery within the last year, because the metabolic landscape post-surgery is too dynamic to layer another agent on top without careful thought
The oligo library was cloned as previously described (Joung et al., 2017) into lentiCRISPRv2, (Addgene cat#52961)
If you add the same 2ml of bacteriostatic water, you get a concentration of 5mg/ml instead of 2.5mg/ml
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