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pH value and viscosity adjustment pH value adjustment: The pH value of the cream needs to be adjusted to an appropriate range (usually 5.5-7.0) to conform to the physiological environment of the skin and reduce irritation

For easier dosing calculations, many practitioners use: 2 mg vial + 2 mL bacteriostatic water = 1 mg/mL (1,000 mcg/mL) 5 mg vial + 2 mL bacteriostatic water = 2.5 mg/mL (2,500 mcg/mL) Step 3: Draw Bacteriostatic Water Clean the rubber stopper of the bacteriostatic water vial with an alcohol prep pad

The qRT-PCR protocol included preincubation at 95 C for 30 s for 1 cycle

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