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Meanwhile, NNMTi preserves lean body mass: it reduces fat accumulation without causing muscle loss, which is crucial for maintaining basal metabolism and preventing weight regain after dieting.These in vivo findings fully validate the efficacy and safety of NNMTi in obesity intervention, laying a solid foundation for its subsequent clinical research and drug development

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Further mechanistic studies showed that NNMTi increases intracellular NAD levels and activates the SIRT1 pathway by inhibiting NNMT, thereby suppressing the transcription of adipogenic genes and preventing the maturation of preadipocytes.This research provides a new perspective for understanding the molecular mechanism of adipocyte differentiation, confirms the key role of NNMTi in regulating adipogenesis, and lays a foundation for subsequent obesity intervention studies