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Liver sample (10 mg) was then mixed with K 2 SO 4 (1 g) and a single reference standard of CE (18:0) (250 ng), followed by two extractions with 2 ml of 1:1 methyl tert-butyl ether/hexane

Inhibition of protein translation restored both mTORC1 signaling and the ISR during cystine starvation, suggesting the bulk of cellular cysteine was committed to the biosynthetic process