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1 Steno Diabetes Center Copenhagen, Herlev, Denmark, 2 Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark, 3 Zealand University Hospital, Kge, Denmark, 4 Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark, 5 Clinical Metabolic Physiology, Gentofte Hospital, Hellerup, Denmark

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Data acquisition Exendin-4: Data for the GLP1R:DNGs:exendin- complex was collected on a Titan Krios microscope operated at 300 kV (ThermoFisher Scientific equipped with a Gatan Quantum energy filter operating in Zero Loss mode with an energy slit with of 20 eV and a Gatan K2 Summit direct electron detector (Gatan)