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Four metabolitescoumarin, L-leucine, L-octanoylcarnitine, and kaempferoldemonstrated statistically significant group-wise differences between ASD and control samples (Fig

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The cultured medium was replaced with unlabeled DMEM medium, supplemented to final concentrations of HEPES (10 mM), sodium bicarbonate (30 mM), sodium pyruvate (1 mM), glutamine (1 mM), and glucose (5 mM) for 2 h to equilibrate the metabolite levels in both cell types