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Mutations conferring GSH-independence map to the protomer interface between proteins VP1 and VP3 and are consistent with GSH binding being essential for the formation of pentameric subunits critical to assembly 24,27

Constipation is reported less frequently than nausea or diarrhoea in clinical trials, nevertheless it remains a significant and sometimes persistent concern in real-world use

When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr

Requires measuring: Not as convenient as pre-capsulated options