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FITC-agonist was added for 30 min at 37 C to induce internalization, then removed and washed with cold HBSS to arrest further endocytosis, and cells treated with 500 mM ice-cold sodium 2-sulfanylethanesulfonate (MesNa), a membrane-impermeable reducing agent, in alkaline TNE buffer (100 mM NaCl, 50 mM Tris-HCl, pH 8.6) to cleave BG-SS-biotin specifically from cell surface receptors
Research on Mfn2 gene expression in hepatocellular carcinoma and its antitumor mechanism
The liver is also in charge of regulating blood sugar levels and, unfortunately, cant effectively do both simultaneously
How effective are Semaglutide GLP-1 and Ozempic for weight loss
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