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GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating
One prior report described PSD95-positive elements contacting intestinal enterochromaffin cells of the mouse 39 , but neither provided quantitative data nor proof of staining specificity
Bri (00:53:00): I have not
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