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Chondrocytes were treated with various doses of IGF-1 for 1 h, stimulated with IL-1 for 24 h, then washed twice with PBS and incubated with 5 M CellROX Green reagent in PBS at 37C for 60 min in the dark

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Key post hoc analysis of the landmark Steno-2 trial showing that intensified, multifactorial treatment slows progression of diabetic nephropathy and renal function loss in microalbuminuric patients with T2DM