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This ultra-short pulse duration produces a predominantly photoacoustic effect (mechanical shattering of melanin) rather than a photothermal effect (heat), resulting in significantly lower risk of heat-induced post-treatment pigmentation compared to older Q-switched lasers

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From measurement of melanin contents, 1N hot NaOH (70C) was used to and dissolved the melanocyte pellets for 1 hour and further centrifuged at 10,000 g for 10 min