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CLO was co-administered with CY to the rats in an attempt to mitigate the lung-damaging effects of the CY because CLO contains eucalyptol (44.34%), which has been reported to possess mucolytic, cytotoxic, antioxidant, and anti-inflammatory properties ( In lung tissues, eucalyptol (10 mg/mL) decreased the elevated pulmonary MDA level induced by cigarette smoking ( in vitro GSH and CAT levels, which were decreased by the cigarette extract on the human bronchial epithelial cell line (BEAS-2B) ( In addition to eucalyptol, CLO also contains -pinene (31.41%), which demonstrated potent antioxidant properties, the ability to scavenge DPPH-free radicals at 12.57 mg/mL, and a high reducing power of 213.7 g/mL in converting the ferricyanide complex to the ferrous form (Wang et al., 2019)

The physicochemical characteristics of the liposomes, including particle size, polydispersity, and zeta potential, were later analyzed as previously described (de Castro et al