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The supernatant was then analyzed in duplicate by sandwich ELISA using the R&D Systems Bovine IL-6 assay (R&D Systems, Minneapolis, USA) following the manufacturers protocol
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reported that in an IRI mouse model, CEUS-measured renal perfusion significantly decreased after one hour and was negatively correlated with renal function recovery [143]
Moreover, free-ranging animals might also have been exposed to concomitant stressors that would strengthen the detrimental effects of a viral infection
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