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[64], which involved a co-culture of primary astrocytes with the bEnd.3 endothelial cell line, incubation with exendin-4 reduced the levels of the astrocyte-derived vascular endothelial growth factor (VEGF-A), matrix metalloproteinase-9 (MMP-9), chemokine monocyte chemoattractant protein-1 (MCP-1), and the chemokine C-X-C motif ligand 1 (CXCL-1), induced by oxygenglucose deprivation (OGD), and subsequently led to the increased expression of tight junction proteins in the bEnd.3 cells

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Because U2OS cells do not express detectable procaspase-1, this result supports the idea that X(PC) can engage off-target apoptotic signaling when its intended substrate is absent