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Extensive investigations of G6PD from G6PD-deficient cells, mostly carried out before the sequence of G6PD was known, revealed that (1) enzyme activity, even when severely reduced (sometimes to less than 1% of normal), is never completely absent and (2) enzymic properties ( K m , K i , activity on substrate analogues, and thermostability, for example) are often different from those of the normal enzyme (i.e., G6PD deficiency is associated with qualitative abnormalities)
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the identification of neurons that express these enzymes in the rodent cerebral cortex, hippocampus, olfactory bulb, amygdala, and thalamus suggests that ALLO and THDOC can be synthesized locally from precursors within the CNS (94)
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