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For CBB staining, SDS-PAGE gels were stained for 10 min using staining solution (0.1% Coomassie Brilliant Blue G250, 10% acetic acid, 50% methanol, and 40% H 2 O 2 ) and destained with distilled water overnight

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Injection technique follows the same principles covered in our retatrutide injection guide and GLP-1 injection guide