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ACC 57) at passage 425 were cultured in Dulbeccos Modified Eagle Medium (DMEM) (Gibco, Ref: 41966029, Thermofisher Scientific,Waltham, Massachusetts, United States) supplemented with 10% foetal bovine serum (FBS) (Gibco, Ref: 10270106, Thermo Fisher Scientific, Waltham, Massachusetts, United States) in humidified air at 37 C and 5% CO 2

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Malaguarnera, M., Vacante, M., Motta, M., Giordano, M., Malaguarnera, G., Bella, R., Nunnari, G., Rampello, L., and Pennisi, G