In pulmonary fibrosis studies using bleomycin to induce lung injury in mice, GHK-Cu doses of 0.0026 to 0.26 mg/mL/day administered every other day reduced inflammatory cell infiltration, decreased interstitial thickness (the scarring that stiffens lung tissue), and lowered TNF-alpha and IL-6 expression
Interestingly, the percentage increase in Collagen I between the control and DCM cohorts was observed at 436% (SMD 2.31, 95%CI 0.62, 3.99, p = 0.007) and 276% for Collagen III (SMD 2.43, 95%CI 0.59, 4.27, p = 0.010)
By extracting aortic tissue from the mice and performing WB analysis, we found that the expression levels of P53 and FOXO4 were both elevated (Supplementary Figure S1A)
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