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Sleep in itself makes no difference, the only benefit being that the time you are asleep gives your liver the opportunity to metabolise the alcohol
Additionally, its important not to leave the water open for long periods of time as this may cause bacteria or other contaminants to enter the solution
This extended trial period (versus typical 30-60 day windows) acknowledges that tissue repair processes are gradual and that fair assessment requires adequate time
Drug Metab Dispos 42:2832 Coles B, Wilson I, Wardman P, Hinson JA, Nelson SD, Ketterer B (1988) The spontaneous and enzymatic reaction of N-acetyl-p-benzoquinonimine with glutathione: a stopped-flow kinetic study
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