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In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min

Reaction of protein thiols with low-molecular weight thiols such as glutathione (GSH) can yield mixed disulfides

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Plasminase promotes the conversion of matrix metalloproteinases to active metalloproteinases at 30 min after PHx (Kim et al., 2000)