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After washed twice with PBS in the plates, cells were removed from the plate by scraping twice with each 0.3 ml BHM solution consisting of 0.32 M sucrose, 1 mM EDTA, 10 mM Tris-Cl (pH 7.0) and the complete protease inhibitor cocktail (Roche Applied Science, Upper Bavaria, Germany)

Expecting overnight results

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7 Adverse effects of exogenous testosterone use 7.1 Cardiovascular toxicity Chronic exposure to supraphysiological concentrations of anabolicandrogenic steroids (AAS) has been associated with systemic toxicity affecting cardiovascular tissues