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One further detail separates the most thorough certificates from the rest: whether the laboratory assayed multiple vials from the lot independently or ran a single composite sample
Analysis of single-cell RNA sequencing Single-cell RNA sequencing (scRNA-seq) data (HRA000425) were downloaded from Genome Sequence Archive (SRA)-Human databases in China National Center for Bioinformation 16 , converted into FASTQ format using fasterq-dump software (sratoolkit 3.0.2, NCBI, NIH, Bethesda, MD, USA), converted into a matrix data file using Cell Ranger software version 3.0.2 (10xGenomics, Pleasanton, CA, USA), converted into Seurat objects using Seurat 5.0.1 software (Satija lab, Lower Manhattan, New York, NY, USA), and plotted for t -distributed stochastic neighbor embedding ( t -SNE) visualization
doi: 10.1016/j.jds.2024.09.021 180 RuanJWeiXLiSYeZHuLZhuangRet al
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