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Purified complex (3 L, ~4 mg/mL) was applied to the grids that were subsequently vitrified in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) under 100% humidity at 4 C
If visible rubber fragments appear in the vial contents after a draw, the documented standard is to discard the vial regardless of remaining volume or days left in the window
Deris, Z
Glutathione production by the #14 mutant was tested in YCK50D and media composed of 10% (v/v) molasses and varying CSL concentrations (0%, 5%, 10%, 15%, 20%, and 25% v/v)
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