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Cells were imaged in fresh FluoroBrite DMEM supplemented with 4 mM glutamine, 1 mM sodium pyruvate, 10% FBS and penicillin (100 U ml 1 )/streptomycin (100 g ml 1 )/fungizone (0.25 g ml 1 )
This is a reversible modification and adding sodium borohydride (NaBH4) results in the GSH release, which can subsequently react with DTNB and be measured
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However, there is a second theory that proposes that gluten's effects on gut lining cells are the primary cause
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