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Consistently supplementing is important, even on days when you are not training, says Jillian

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In Vitro Embryo Culture The presumptive zygotes were denuded from cumulus cells by gentle pipetting and cultured in 50 L droplets in CR2aa medium supplemented with 1 mg/mL BSA, 40 g/mL sodium pyruvate, 5 mg/mL hemi-calcium, 20 L/mL amino acid solution (0.09 g/mL glutamine, 0.15 g/mL alanine, and 0.75 g/mL glycine), 20 L/mL Eagle Basal Medium (EBM), 10 L/mL Eagle Minimum Essential Medium (MEM) and 5% FBS, at 38.5C in a humidified incubator in air with 5% CO2

Independent samples t -test was used to compare the normoxic and hypoxic groups, and two-way ANOVA was used to test the main effects of glutathione level, dissolved oxygen, and their interaction effects