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Blood and tissue processing For all studies blood was drawn by cardiac puncture in anesthetized mice and collected in 5 l EDTA (BD medical) before being spun in a microcentrifuge at 9.8 revolutions per minute (RPM) for 5 min at room temperature

Manufacturer-supplied solutions of horseradish peroxidase, Amplex Red, and lactate oxidase were added to samples in wells of a 96-well microplate which was then incubated at room temperature for 30 min

Ipamorelin is a selective ghrelin receptor agonist and GH secretagogue, often combined with CJC 1295 in unregulated products without robust clinical evidence supporting this combination

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